Clontech talon resin manual

TALON® resin. For more information on these and other Creator vectors, please refer to Tables I and II or visit the Clontech page at www.doorway.ru Complete Systems compatible with Creator are available for several of our gene expression systems. These complete kits offer a streamlined approach to express-ing your target gene. TALON Superflow is used to purify his-tagged proteins from prokaryotic or eukaryotic expression systems. It is suitable for purification under native or denaturing conditions, and it can bind up to 20 mg of his-tagged protein per mL resin. The resin is compatible with commonly used IMAC reagents. Read more. His-tagged proteins sometimes require. Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT–1 A Takara Bio Company Version No. PR8Z 3 HisTALON™ Cartridge Purification Kit User Manual I. Introduction Clontech’s HisTALON™ Cartridges are ready-to-use columns for the efficient purification of his-tagged proteins, using automated or syringe-based protocols, from bacterial, mammalian, and .

Clontech Laboratories, Inc. • www.doorway.ru 3 TALON Resin and Magnetic Beads TALON Resin • Use for batch/gravity applications • Highest purity of your target protein TALON Superflow Resin • Use for FPLC applications • Highest purity of your target protein TALON CellThru Resin. Protocol-at-a-Glance (PT) and the indicated sections of the TALON Metal Affinity Resins User Manual (PT), which are available at www.doorway.ru Spin Column Batch Batch/Gravity-Flow Method (PT) (P T, Sec. V.B) (PT, Sec. V.A) Binding Conditions Resin bed-volumea ml ml ml. TALON® Metal Affinity Resins User Manual ommended conditions, even in the presence of strong denaturants such as 6 M guanidinium. Such durability allowsT ALON Resin to be reused (See Section IX). Cobalt IMAC Resin permits milder elution conditions TALON Resin exhibits subtle yet important differences in binding of polyhis-.

The protocol includes instructions for making the required buffers as well as TALON™ is a registered trademark of Clontech Laboratories Inc., USA. Clontech Laboratories, Inc. TALON. ®. Metal Affinity. Resins User Manual TALON CellThru Resin is a novel IMAC resin for purifying his-tagged proteins. The Co2+–CMA matrix (Talon resin, available from Clontech) has a somewhat lower affinity for the polyhistidine affinity tag than the Ni2+–NTA resin.